IHC staining of FFPE human breast cancer with RICTOR antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Immunofluorescent staining of FFPE human A431 cells with RICTOR antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Western blot testing of human 1) U-2 OS, 2) K562 and 3) PC-3 lysate with RICTOR antibody. Predicted molecular weight ~200 kDa.

Flow cytometry testing of human 293T cells with RICTOR antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RICTOR antibody.